(Not for data entry)
The ethics project/protocol number as given by the ACEC.

(Not for data entry)
The ethics application number.

(Not for data entry)
The full project title as it appears on the Ethics Application.

Please mark appropriate box

A four-figure Statistical Reference Number (SRN) is issued to each accredited establishment by NSW Agriculture and should be entered in this column as follows:

2485 UNSW
2485a Liverpool Hospital
2485b Prince of Wales Hospital
2485c Sydney Children’s Hospital
2485d St George Hospital

Select Yes which will open the rest of the questions; No will direct the form to the last section.

Enter the most appropriate from those listed below to describe the primary purpose of the project.

1. Stock breeding
   Breeding protocols to produce new teaching or research stock. Include the animals used to produce progeny and any breeders or progeny culled in the process, NOT the final progeny themselves (as these will be counted under the protocol in which they go on to be used).
   



2. Stock maintenance
   Holding protocols for animals maintained for use in other protocols. These animals may be maintained under an ethics authority because they require special management. If they are not held under an authority, (eg. normal stock animals kept mainly for commercial production, but occasionally used in research) then they are only counted in the protocol where they are used for teaching/research.
   
   Examples
   • Fistulated ruminants which are maintained under a holding protocol, for use in other short term feeding trial protocols
   • Non-breeding colony of diabetic rats held for research in other protocols
   



3. Education
   Protocols carried out for the achievement of educational objectives. The purpose of the protocol is not to acquire new knowledge, rather to pass on established knowledge to others. This would include interactive or demonstration classes in methods of animal husbandry, management, examination and treatment.
   
   Examples
   • Animals used by veterinary schools to teach examination procedures such as pregnancy diagnosis
   • Sheep used in shearing demonstration classes for students; Dogs used to teach animal care to TAFE students
   



4. Research: human or animal biology
   Research protocols which aim to increase the basic understanding of the structure, function and behaviour of animals, including humans, and processes involved in physiology, biochemistry and pathology.
   



5. Research: human or animal health and welfare
   Research protocols, which aim to produce improvements in the health and welfare of animals, including humans.
   



6. Research: animal management or production
   Research protocols which aim to produce improvements in domestic or captive animal management or production.
   



7. Research: environmental study
   Research protocols which aim to increase the understanding of the animals’ environment or its role in it, or aim to manage wild or feral populations. These will include studies to determine population levels and diversity and may involve techniques such as observation, radio tracking or capture and release.
   
   Examples
   • Pre-logging or pre-development fauna surveys
   



8. Production of biological products
   Using animals to produce products other than milk, meat, eggs, leather, fur, etc.
   
   Examples
   • Use of a sheep flock to donate blood to produce microbiological media
   • Production of commercial anti-serum
   • Production of products, such as hormones or drugs, in milk or eggs from genetically modified animals
   • Quality Assurance testing of drugs but do not include animals which come under code 10, below.
   



9. Diagnostic procedures
   Using animals directly as part of a diagnostic process.
   
   Examples
   • Inoculation of day old chicks with ND Virus to determine virulence
   • Blue-green algae toxicity testing
   • Water supply testing using fish
   



10. Regulatory product testing
    Protocols for the testing of products required by regulatory authorities, such as the National Registration Authority. If the product testing is not a regulatory requirement, eg. it is part of a quality assurance system only, those animals should be included in the appropriate category selected from above. (This would be normally be category 8 in the case of QA testing.)
    
    Examples
    • Pre-registration efficacy or toxicity testing of drugs and vaccines
    

Enter the highest appropriate numerical code (1-9) from those listed below to describe the type of procedures carried out on the animals in the project. The descriptions given are a guide only.

Where 'Death as an endpoint' or 'GMO production' applies, animals must be placed in these categories (8 or 9) rather than any others which might also appear appropriate.

1. Observation Involving Minor Interference
   Animals are not interacted with or, where there is interaction, it would not be expected to compromise the animal's welfare any more than normal handling, feeding, etc. There is no pain or suffering involved.
   
   Examples
   • Observational study only
   • Breeding animals for supply, where only normal husbandry procedures are used
   • Breeding or reproductive study with no detriment to the animal
   • Feeding trial, such as Digestible Energy determination of feed in a balanced diet
   • Behavioural study with minor environmental manipulation
   • Teaching of normal, non-invasive husbandry such as handling, grooming, etc
   • Production of products, such as hormones or drugs, in milk or eggs from genetically modified animals which are subject to normal husbandry procedures only
   



2. Animal Unconscious Without Recovery
   Animal is rendered unconscious under controlled circumstances (i.e. not in a field situation) with as little pain or distress as possible. Capture methods are not required. Any pain is minor and brief and does not require analgesia. Procedures are carried out on the unconscious animal which is then killed without regaining consciousness.
   
   Examples
   • Laboratory animals killed painlessly for dissection, biochemical analysis, etc
   • Teaching surgical techniques on live, anaesthetised patients which are not allowed to recover following the procedure
   



3. Minor Conscious Intervention
   Animal is subjected to minor procedures which would normally not require anaesthesia or analgesia. Any pain is minor and analgesia usually unnecessary, although some distress may occur as a result of trapping or handling.
   
   Examples
   • Injections, blood sampling in conscious animal
   • Minor dietary or environmental deprivation or manipulation, such as feeding nutrient-deficient diets for short periods
   • Trapping and release as used in species impact studies, etc
   • Trapping and humane euthanasia for collection of specimens
   • Stomach tubing, branding, disbudding, shearing, etc
   



4. Minor Surgery With Recovery
   Animal is rendered unconscious with as little pain or distress as possible. A minor procedure such as cannulation or skin biopsy is carried out and the animal allowed to recover. Depending on the procedure, pain may be minor or moderate and post-operative analgesia may be appropriate.
   
   Field capture using chemical restraint methods is also included here.
   
   Examples
   • Biopsies
   • Cannulations
   • Sedation/anaesthesia for relocation, examination or injections/blood sampling
   



5. Major Surgery With Recovery
   Animal is rendered unconscious with as little pain or distress as possible. A major procedure such as abdominal or orthopaedic surgery is carried out and the animal allowed to recover. Post operative pain is usually considerable and at a level requiring analgesia.
   
   Examples
   • Orthopaedic surgery
   • Abdominal or thoracic surgery
   • Transplant surgery
   • Mulesing, castration without anaesthesia
   



6. Minor Physiological Challenge
   Animal remains conscious for some or all of the procedure. There is interference with the animal's physiological or psychological processes. The challenge may cause only a small degree of pain/distress or any pain/distress is quickly and effectively alleviated.
   
   Examples
   • Minor infection, minor or moderate phenotypic modification, early oncogenesis
   • Arthritis studies with pain alleviation.
   • Prolonged deficient diets, induction of metabolic disease.
   • Polyclonal antibody production
   • Antiserum production
   



7. Major Physiological Challenge
   Animal remains conscious for some or all of the procedure. There is interference with the animal's physiological or psychological processes. The challenge causes a moderate or large degree of pain/distress which is not quickly or effectively alleviated.
   
   Examples
   • Major infection, major phenotypic modification, oncogenesis without pain alleviation
   • Arthritis studies with no pain alleviation, uncontrolled metabolic disease
   • Isolation or environmental deprivation for extended periods
   • Monoclonal antibody raising in mice
   



8. Death As An Endpoint
   This category only applies in those rare cases where the death of the animal is a planned part of the procedures. Where predictive signs of death have been determined and euthanasia is carried out before significant suffering occurs, they may be placed in category 6 or 7.
   
   Examples
   • Lethality testing (LD50, LC50)
   It does not include: death by natural causes; animals which are euthanased on completion of the project; animals which are killed if something goes wrong; animals killed for dissection or for use as museum specimens; or accidental deaths.
   



9. Production of genetically modified animals
   This category is intended to allow for the variety of procedures which occur during the production of genetically modified animals. As animals in this category may be subjected to both minor and major physiological challenges and surgical procedures, this category reflects the varied nature of the procedures carried out. It Effectively includes ALL animals used in GM production other than the final progeny which are used in a different category of procedure.
   
   Examples
   • Initial breeding animals for GM production
   • Animals culled as part of the GM production process
   

Enter the numerical code (1-56) from those listed below to describe the species or species group used in the project; number of animals that were actually used (i.e. not just the number supplied or authorised) in the protocol in the year for which statistics are being collected; and number of animals that were held for 12 months or longer in this project

Use this column to communicate any other information you think may be relevant.

Indicate whether the conduct of the project complies with the NSW Animal Research Act, its Regulations and Code, and any stipulations in the approval letter from the ACEC.

Briefly describe the progress the project has made. It is acceptable to attach a list of publications and/or conference presentations arising from this project.

Indicate what measures, if any, you have taken to refine, reduce or replace the use of animals in this project. Be sure to describe any unanticipated adverse consequences of your procedure that arose in the past year and the measures taken to redress them. Please note that any severe unanticipated consequences should be reported immediately to the ACEC.

This section is only for researchers using procedures listed.
Complete with the species, number of animals used in 2008 and total number of animals used to 31 December 2008.

(Not for data entry)
This section will list the Chief Investigator who completes the form and the date of completion.